Formula one Qi (Ziehl) Carbolic acid magenta dye solution Take 5g of carbolic acid and dissolve it in 95 ml of distilled water. Take 0.3g of basic fuchsin in liquid B, grind it in a mortar, gradually add 10 ml of 95% alcohol, and continue to submerge to dissolve it.

1 Bacterial stain

Formula oneZiehl Carbolic acid magenta dye solution

Take 5g of carbolic acid and dissolve it in 95 ml of distilled water.

Take 0.3g of basic fuchsin in liquid B, grind it in a mortar, gradually add 10 ml of 95% alcohol, and continue to submerge to dissolve it.

After mixing liquid A and liquid B, shake well, filter and bottle for later use.

 

Formula IIRoche (Loeffler's) methylene blue dye solution

Take 5g of methylene blue in a solution and dissolve it in 100 ml of 95% alcohol to make methylene blue-alcohol saturated solution.

Take 0.01g of potassium hydroxide (or 1 ml of 1% potassium hydroxide solution) for liquid B. the solution can also be used for actinomycetes staining, and 0.1 concentration can be used for yeast staining.

 

Formula threeGram's Dye Solution

Dyeing with this solution is done by first dyeing with solution A, then fixing with solution B, treating with solution C, and finally counterdyeing with solution D. The formula of the four liquids is as follows:

Liquid A (crystal violet solution)

(1) Crystal Violet 2g 95% alcohol 20 ml

(2) ammonium oxalate 0.8g distilled water 80 ml

Qianjiang (1) and (2) were mixed in liquid phase and allowed to stand for 48 hours before use.

Liquid B (iodine solution)

Iodine 1g potassium iodide 2g distilled water 300 ml

Dissolve potassium iodide in a small amount of distilled water, then add iodine, and after all iodine is dissolved, add water to dilute to 300 ml.

95% alcohol solution

Ding liquid (saffron red liquid)

Distilled water 100 ml 2.5% saffron red alcohol solution 10 ml

 

2 Bacterial special stain

Formula onespore dyeing liquorDyeing with this formula is followed by staining with A solution and counterstaining with B solution.

Take 5g of malachite green, add a small amount of distilled water, dissolve it, and dilute it to 100 ml with distilled water to form malachite green dye solution.

Take 0.5g of crocus red in liquid B, add a small amount of distilled water to dissolve it, dilute it to 100 ml with distilled water, and integrate crocus red re-dyeing solution.

 

Formula IIcapsule dye solutionThis formula is first dyed with liquid A and then counterdyed with liquid B.

0.1g of crystal violet is taken from liquid a, dissolved in a small amount of distilled water, diluted to 100 ml with water, and then 0.25 ml of glacial acetic acid-crystal violet dye solution is added.

Take 31.3g of copper sulfate in liquid B, dissolve in a small amount of distilled water, and dilute to 100 ml with water to form 20% copper sulfate decolorant.

 

Formula threeflagellum dye solution

A liquid

Saturated alum solution 2 ml 5% carbolic acid solution 5 ml

20% tannic acid solution 2 ml

Liquid B

Basic fuchsin 11g 95% alcohol 100 ml

Before use, mix 9 ml of liquid A and 1 ml of liquid B and filter.

 

3 Plant Cell Wall Stain

Formula oneCELLULOSE CELL WALL DYE (Ⅰ)

Take 0.1g of solid green and dissolve it in 100 ml of 95% alcohol to form 0.1 solid green-alcohol solution.

The solution can dye cellulose cell walls, but also used in animals and plants as a pulp stain.

 

Formula IIDye Solution for Cellulose Cell Wall (Ⅱ)

Zinc chloride 20 grams potassium iodide 6.5 grams

Iodine 1.5g distilled water to 100 ml

First, zinc chloride is dissolved in a small amount of distilled water, and then 6.5 grams of potassium iodide is added. After iodine is completely dissolved, it is diluted to 100 ml with distilled water to form iodine-zinc chloride solution.

The dye can dye the cell wall purple, the cytoplasm pale yellow, and the nucleus brown.

 

Formula threeCellulose Cell Wall Dye Solution (Ⅲ)

Take 1g of iodine and 1.5g of potassium iodide and dissolve them in 100 ml of distilled water to form 1% iodine solution.

7 parts of sulfuric acid and 3 parts of distilled water are mixed in liquid B to form 66.5% sulfuric acid solution.

When dyeing, add a drop of liquid A and a drop of liquid B on the material, and the cellulose cell wall will be dyed yellow.

 

Formula fourLignified cell wall dye (Ⅰ)

Sulfated aniline (or hydrochloric acid) 1 part

Distilled water 70 parts 95% alcohol 30 parts

Sulfuric acid 30 parts

The above components are mixed, and the cell material is stained in the mixture, but the lignified cell wall is bright yellow or turmeric.

 

Formula 5Lignified cell wall dye (Ⅱ)

Take 4~5 grams of phloroglucinol, dissolved in 100 ml of 95% alcohol, that is, phloroglucinol-wine semen.

First drop 1 drop of concentrated hydrochloric acid on the material, then drop 1 drop of phloroglucinol-wine semen, and the lignified cell wall is stained with cherry red or purple red.

 

Formula 6Lignified cell wall dye (Ⅲ)

Take 1g of safranin and dissolve it in 99 ml of distilled water to form a 1% safranin solution.